Bodipy pepstatin a
WebThe BODIPY FL-pepstatin A is a potent inhibitor for cathepsin D. It inhibits the protease activity of cathepsin D with an IC 50 ~50 nM; 2. The binding of BODIPY FL-pepstatin A … WebTo test whether neuronal LAMP1-labeled organelles represent acidified structures containing activated hydrolases, we used boron-dipyrromethene (BODIPY)-modified full-length (FL)–pepstatin A, a fluorophore-tagged pepstatin that specifically binds active cathepsin D in acidic environments at pH 4.5.
Bodipy pepstatin a
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WebPLA enhanced binding of Bodipy-pepstatin-A to the active site of cathepsin D in compromised cells. PLA also reduced the cellular levels of opsin and the lipofuscin-like … WebMar 16, 2000 · In vitro, BODIPY FL-pepstatin A inhibits cathepsin D activity with an IC50 of 10 nM. The nature of its binding to cathepsin D was further characterized using a …
Web... neurons with BODIPY FL pepstatin A. For this probe, the CatD-specific inhibitor pepstatin A has been conjugated with a pH-insensitive fluorophore ( Chen et al., 2000). NHE6-null neurons... WebMar 16, 2000 · In vitro, BODIPY FL–pepstatin A inhibits cathepsin D activity with an IC 50 of 10 nM. The nature of its binding to cathepsin D was further characterized using a …
WebCatalog number: P12271. For the study of cathepsin D trafficking in cells by fluorescence microscopy, try our BODIPY FL pepstatin A. This green-fluorescent probe, which … WebTag:BODIPY FL-pepstatin A Related Product Information Pepstatin According to the laws, regulations and policies related to " patent products ", the sale of this product is prohibited!
WebJul 2, 2024 · BODIPY-FL-pepstatin A (1 μM) was applied for 30 minutes in both the soma and axon chambers of microfluidic devices containing DIV7-8 cortical neurons in order to label active cathepsin D-containing lysosomes. Time-lapse sequences were collected at 2 s intervals for a total of 120 frames. Distal axons are toward the right.
WebBODIPY™ FL pepstatin A, a green-fluorescent probe, which inhibits cathepsin D in vitro (IC50 approx. 50nM), targets cathepsin D within lysosomes of live cells. Initial studies … the pearl of the mediterraneanWebConfocal microscopy single-plane images of BODIPY-pepstatin A colocalization with different endosomes and the lysosome in male WT and NHE6-null mouse primary hippocampal neurons at DIV 5 and 14. the pearl on frankford dallasWebDec 1, 2024 · The colocalization of BODIPY pepstatin A and LysoTracker Red was enhanced by TSA, suggesting that the new lysosomes were mature and functional ( Fig. 1, H and I ). As a transcription factor, TFEB promotes the expression of autophagic and lysosomal genes. Therefore, we examined the expression of TFEB target genes. the pearl of the seaWebMar 16, 2000 · Synthesis of BODIPY FL–pepstatin A Pepstatin A (25 mg, 0.036 mmole) was first dissolved in a solvent containing DMSO and DMF (2 ml of each). To this … sial architektiWebOther suitable methodologies rely on the use of fluorescently labelled inhibitors of lysosomal hydrolases such as Bodipy FL-Pepstatin A (Invitrogen, P12271), a … the pearl of the indian oceanWebMay 25, 2011 · Neurons plated on 35 mm glass-bottom chamber dishes (BD Biocoat) were incubated with 1 μ m of either Bodipy-pepstatin-FL (Invitrogen), Magic Red cathepsin B (Marker Gene Technologies), or LysoTracker Red (Invitrogen) in Neurobasal medium for 30–60 min at 37°C followed by washing with fresh Neurobasal medium (two times). sia largest it staffing firms 2021WebA mature degradative lysosome is defined as: (A) a storage organelle for active forms of degradative enzymes with acidic pH optimum, allowing substrate degradation; (B) limiting membranes with specific glycosylated membrane-associated proteins such as LAMP1/2; and (C) lacking non-lysosomal proteins such as IGF2R/CI-M6PR (insulin like growth … sia largest healthcare staffing firms 2022