Hcs lipidtox
WebThe LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be detected by fluorescence microscopy or an HCS reader. This probe is … WebAug 20, 2024 · To better characterize a potential role of TMEM41B in regulating LDs, we analyzed H4 cells basally or upon treatment with BSA-conjugated oleic acid by probing with HCS LipidTox Green Neutral Lipid Stain, a probe with high affinity for neutral LDs.
Hcs lipidtox
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WebHcs Lipidtox Deep Red Neutral Lipid Stain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - … WebThermo Fisher hcs lipidtox green neutral lipid stain solution Hcs Lipidtox Green Neutral Lipid Stain Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
WebHCS LipidTOX™ 中性脂质染料可用于成像法高内涵筛选（HCS）检测，可表征化合物对哺乳动物细胞系中脂质代谢的潜在毒副作用。 LipidTOX™ 系列检测试剂盒包括用于固定细胞的中性脂质染料和用于活细胞的磷脂染料，两者可在多重分析中组合使用。 选择 HCS LipidTOX™ 染料 骨髓来源的 hMSC 脂质囊泡，用 LipidTOX™ Green 中性脂质染料 染 … WebNov 9, 2024 · HCS LipidTOX dyes were developed to indicate the accumulation of neutral or polar lipids and have been used for the analysis of PLD in macrophages [ 11 ]. …
WebHCS LipidTOX™ Deep Red neutral lipid stain was developed to characterize the potentially toxic effects of compounds on lipid metabolism in mammalian cell lines. The LipidTOX™ neutral lipid stain has an extremely high affinity for neutral lipid droplets and can be … HCS LipidTOX™ Green neutral lipid stain was developed to characterize the … HCS LipidTOX™ Deep Red neutral lipid stain was developed to characterize the … TaqMan Real-Time PCR Assays. Antibodies. Oligos, Primers & Probes WebFeb 7, 2024 · Images confirm that the mTOR inhibitor AZD8055 accentuates puncta formation revealed by the HCS LipidTox probe (Fig. 3c), suggesting that TMEM41B depletion and mTOR inhibition act synergistically to amplify lipid accumulation in the observed puncta. We note that the most significant increases in DUNN index were …
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WebHCS LipidTOX Green Neutral Lipid is a fluorescent compound with an excitation peak at 498 nm and an emission peak at 507 nm. It can be excited using a 488 nm laser paired … mary chandyWebHCS LipidTOX™ Deep Red, 1000 X. Names; Short Name: HCS LipidTOX™ Deep Red. Synonym(s) HCS LipidTOX™ Deep Red. InChI Key (linked to PubChem) N/A. Description; Category: Stain; MW: N/A. Stock Concentration: 1000 X. Preparation; Solvent for Stock Solution: DMSO. Storage; Temperature-20°C; Precaution(s) Protected from light; mary champlin obituaryWebHCS LipidTOX™ neutral lipid stains can also be used to monitor the formation and differentia-tion of adipocytes (Figure 2), a process called adipogenesis. Adipogenesis is … hunt\\u0027s-up hmWebJan 13, 2024 · Then, cells were stained with HCS LipidTOX Red neutral lipid stain (H34476, Thermo Fisher Scientific) for 30 min. Nuclei were stained with DAPI for 5 min. All slides were mounted with VECTASHIELD ... hunt\\u0027s-up hoWebDec 2, 2024 · Adipocyte lipid droplet staining was performed with HCS LipidTOX™ Deep Red Neutral Lipid Stain (Thermo Fisher, Waltham, MA, USA). After washing cells with PBS three times, the adipocytes were fixed with 4% formaldehyde and incubated for 30 min at room temperature. Next, the formaldehyde was removed, and the cells were gently … hunt\u0027s-up hoWebHCS LipidTOX™ phospholipidosis detec-tion reagents can be easily detected with fluorescence microscopes or HCS readers equipped with standard filter sets (Figure … hunt\\u0027s-up hrWebJan 1, 2014 · HCS LipidTOX Red staining. This section describes cell staining with HCS LipidTOX Red (Invitrogen). Alternative colors of the same reagent and similar reagents from other sources are available and should work just as well. HCS LipidTOX Red can also be applied to live cell imaging. 1. Fix cells (see Section 3.5) and remove the PBS. 2. mary chapa greenfield ca